Approach To Estimate The Salivary Flow Using Chromatography

flack To Estimate The Salivary accrue Using ChromatographyAbstractAim To develop a simple and base hit regularity to estimate the spittlery consort using chromatography publisher.Materials and Method 30 volunteers participated in the study. The spittingry come down place was estimated using chromatography diffuse paper and compared with the salivary flow stride per minute. The assay consisted of 3 sight containing stiffen and kB iodide per spot on fall into place paper. ground on the colour in reaction on the chromatography filter paper the salivary flow rate was estimated.Results Pearsons correlation coefficient study showed highly significant negative correlation when the salivary flow rate and the moment of colored dots were compared.Conclusion This rule can be used as a chairside diagnostic helper in affection of salivary flowKey words chromatography filter paper, salivary flow rate, diagnostic aidA Novel Approach To Estimate The Salivary Flow Using Chroma tography PaperIntroduction quantifiable and/or qualitative alte dimensionns in salivary secretion may remove to local unfavorable effects equivalent caries, spontaneous mucositis, candidiasis, oral infections, chewing disorders, halitosis and functional adverse effects like dysphagia, hypersalivation(sialorrhea) and hyposalivation(xerostomia).1Whole saliva (mixed saliva) is a mixture of oral fluids that includes secretions from both the major and minor salivary glands, in addition to some(prenominal) constituents of non-salivary origin, such as gingival crevicular fluid (GCF), expectoratedbronchial and nasal secretions, serum and logical argument derivatives from oral wounds, bacteria and bacterial products, viruses and fungi, desquamated epithelial cells, other cellular components, and regimen debris.2Stimulated saliva is produced on account of some mechanical, gustatory, olfactory, or pharmacologic stimulus, contributing to around 80% to 90% of daily salivary production.In adults the total stimulated salivary flow ranges from 1 to 3 ml/min3whereas the unstimulated salivary flow at rest ranges from 0.25 to 0.35 ml/min.4Unstimulated salivary flow rate is most affected by the degree of hydration, olfactory stimulation, word picture to light, body seting, and seasonal and diurnal factors.There are various orders for the theme of salivary flow including measurement of glandular salivary flow, resting salivary flow and stimulated salivary flow.The best two ways to have totally saliva are the wearing method, in which saliva is allowed to drip cancelled the lower lip, and the spitting method, in which the subject expectorates saliva into a test tube.2This method is cadence consuming and needs special apparatus like collection tubes or volume meter.AIMTo evaluatea simple and safe method for the estimation of salivary flow using chromatography paperMATERIALS AND METHODSThe study consisted of 30 volunteers comprising of 21 females and 9 males, aged 20-2 0yrs. The subjects were asked to sit in an upright position with their head bent in a downward position and were asked to collect the saliva in their gumshield for 1minute and asked to spit into the stack away test tubes at the end of 1 minute to determine the salivary flow rate/min.METHOD OF PREPARATION OF FILTER PAPERThe filter paper for the estimation of salivary flow was prepared according to a method give by Takashi et al using meltable starch, potassium iodideand chromatography paper (70 mm 21 mm).4l of the detection reagent (1% starch solution and 0.3 groin/L potassium iodide solution mixed at a ratio of 31) was placed at 3 places on the chromatography paper using a micropipette.The filter paper was left overnight in a cool dark room and then stored in a light-resistant container until use.The soluble starch and potassium iodide were dissolved in 0.1 mol/L Tris-HCl original the adjusted pH of 7.3.5METHOD OF PREPERATION OF COLORING REAGENTThe coloring reagent was prepare d from a solution of 31% heat content peroxide, ethanol and distilled water at a ratio of 171.METHOD OF COLLECTION OF SALIVASubjects were asked to ease up their mouths and then to lift the tongue.The tip of the paper was put on the snapper of the floor of the mouth in the sublingual region.After 1 minute, the filter paper was taken out and then the coloring reagent (approximately 2 L) was added dropwise to the spots in the filter paper.Via the iodine-starch reaction, colorless spots immediately turned blue.The number of blue spots, including partly colored spots (50%), was grossly counted as an integer.Based on this we examined the relationship between the number of colored spots and salivary flow rates after insertion of filter paper for 1 minute in the sublingual regions of healthy subjects.5DISCUSSIONCommon methods for collecting whole saliva include draining, spitting, sucking and swab (absorbent) method. In draining method saliva is allowed to drip off the lower lip into a p reweighted container or graduated test tube. In spitting method of collection, the saliva is allowed to collect in the floor of the mouth and subjects are asked to spit in the collecting tubes. Whereasin suction method saliva is continuously aspirated from the floor of the mouth into graduated test tubes. In absorbent method pre-weighed cotton rolls, swabs, or gauze are inserted into the opening of the ductal orifices of the salivary glands and reweighed after the collection is completed.The suction and swab method causes some degree of stimulation and variability and then are not recommended for unstimulated salivary collection whereas swab method is say to be least reliable among the above mentioned methods.6 the otherdisadvantages of these methods are that they are time consuming and need special apparatus like collection tubes or volume meter.Takashi et al proposed the present method the present assay for salivary flow assessment. The assay system consists of 3 spots 1mm apart containing starch and potassium iodide on the filter paper. Potassium iodide in the spots well gets displaced with the flow of saliva, in contrast to this starch does not get displaced and gets retained in the original spot. Thus, the colorless spots on the paper not infiltrated with saliva immediately turned blue with the addition of the coloring reagent that contained hydrogen peroxide, whereas the spots on the paper infiltrated with saliva does not show the color. color in spot is based on the reaction of saliva between potassium iodide and starch in the chromatography paper and the color reaction of iodine-starch to hydrogen peroxide.5Unstimulated saliva reflects the basal salivary flow rate while stimulated salivarepresents the fuctional backup of the salivary glands. So the study of unstimulated saliva is useful for the study of salivary gland status.1The presentmethod can be of help in case of aged patients suffering from xerostomia, in screening tests, post radiation th erapy for cancer in elderly patients where salivary flow measurement can play a chair side diagnostic test.In addition, the method can comfortably be adjusted to variations in cutoff values and accuracy by changing the number of spots and distance between the spots on the filter paperWith the present method excellent results were obtained between the salivary flow rate and the colored spots with an r value of -1 and p value of 0.0 in healthy individuals. Thus the routine use of this method as a chair side assessment assay for salivary flow would be of benefit to all practicing dentists and patients alike.